抄録
Callus was induced from Camptotheca acuminata, which produces an antitumor alkalid, camptothecin. Using the Murashige and Skoogs' medium as the basal, cultural conditions were examined for C. acuminata suspension cultures. As a result, a medium, containing 0.1mg/liter 2, 4-D, 3mg/liter kinetin and 0.05mg/liter GA3, was established as a medium that gave the best cell growth in suspension cultures. In addition, conditioning of medium and addition of 0.115mM L-Trp and L-Phe to medium promoted remarkably growth of cell suspensions.
