Coagulation of DNA Polymerase II Isolated from Membrane Fraction of Escherichia coli

Abstract

The coagulating properties of DNA polymerase II were investigated using partially purified enzyme. DNA polymerase II did not coagulate at NaCI concentrations higher than 0.3M but coagulated very rapidly at concentrations lower than 0.2M. The coagulation proceeded at 6 hr after the removal of NaCl. DNA and membrane components did not participate in the coagulation of the enzyme. DNA polymerase II coagulated by itself to form oligomer, and the coagulated enzyme did not dissociate by treatment with 0.3M NaCl or 4M urea. Properties of the coagulated enzyme were the same as the uncoagulated enzyme except for heat stability; coagulated enzyme was rather stable to heat treatment.