Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Effects of Salt Concentration and pH on the Absorption Spectra of Polynucleotides with Various S-Values
Yoshiki KATOHAkira KUNINAKAHiroshi YOSHINO
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1975 年 39 巻 10 号 p. 1957-1962

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Poly (A) took stable double-stranded form in 0.02M phosphate buffer pH 5.8 when its S-value was higher than 3.5. Poly (C) also took stable double-stranded form in 0.01M acetate buffer pH 3.9 containing 0.1M NaCl if its S-value was higher than 4.8.
Poly (I) with S-values higher than 6.5 stably formed triple-stranded form. Poly (A) with S-values over 3 S and poly (I) with S-values over 7 S were observed to aggregate in the presence of high concentration of salt, but poly (C) and poly (U) did not aggregate under the same condition. Effect of salt concentration differed with different polynucleotides and with different salts.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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