Isolation of High Phytin Containing Particles from Rice Grains Using an Aqueous Polymer Two Phase System

Abstract

High phytin containing particles were isolated from rice bran by a combination of a differetial centrifugation and an aqueous two phase system, using dextran 500 and polyethylene glycol 6000. The isolated particles consisted mainly of phytic acid, potassium and magnesium. The chemical composition and electron microscopic observation of the isolated particles confirmed that the electron dense material, which is embedded in aleurone particles of matured rice grains, is potassium and magnesium salts of phytic acid.