Abstract
Cladosporium sp. No. 45-2, an acid protease-producing microorganism, was cultured in medium containing a microbial acid protease inhibitor (S-PI). By the addition of S-PI, the amount of acid protease in the culture broth showed an increase of 50_??_80%. over those of normal culture (S-PI-free). Acid protease was purified from the S-PI-added culture filtrate, and its enzymatic and physicochemical properties were compared with those of acid protease obtained from normal culture. It was determined that the acid protease obtained from S-PT-added culture was the same as that of normal culture, but that the productivity was increased by the addition of S-PI.
The increase in acid protease productivity is assumed to be due to a change in metabolism by the addition of S-PI.
