Interaction between Anti-Sarcoma 180 Serum and Various Lines of Tumor Cells

Abstract

When mice were immunized with adequate doses (1.0_??_5.0mg) of tumor cells attenuated with acetone-ether, complete resistance to the graft of Sarcoma 180 could be induced. The serum taken from mice immunized with repeated challenges was found to display immune adherence reactivity and the antibody titer of anti-Sarcoma 180 serum was higher than that of anti-sarcoma 37 or anti-Ehrlich serum prepared from respective tumor resistant mice.
The interaction between anti-Sarcoma 180 serum and various lines of tumor cells was investigated by the tests of immune adherence absorption and cytotoxicity.
Sarcoma 37 cells exhibited the same reactivity as Sarcoma 180 cells in both tests. Ehrlich cells showed lower reactivity than Sarcoma 180 or Sarcoma 37. Neither MH 134 cells nor myeloma cells exhibited a detectable reactivity in the test of cytotoxicity in vitro. On the other hand, in the test of cytotoxicity in vivo, MH 134 was slightly inhibited and myeloma was promoted in tumor growth.
These results suggest that anti-Sarcoma 180 serum prepared in this experimental system might be useful for the classification of tumor cells and in the study of tumor surface.