Abstract
Among mouse antibodies against Sarcoma 180, immune adherence activity was found only in IgM fraction and cytotoxicity was recognized in fractions both IgM and IgG. Hyperimmunization of mouse with Sarcoma 180 cells increased the cytotoxicity in some fractions of IgG and also brought about an appearance of a new protein (protein T) with immune adherence activity. A molecular weight of the new protein as estimated by gel filtration was approximately 240, 000.
On reduction and carboxymethylation, cytotoxic activity of IgM and immune adherence activity of IgG disappeared completely. Contrary to this, considerable retentions of cytotoxicity of IgG and immune adherence activity of IgM were observed.
