4-O-Methylglucuronoxylan Isolated from the Midrib of Nicotiana tabacum

Abstract

An acidic xylan from the midrib of Nicotiana tabacum was isolated by alkaline extraction and fractionation on a DEAE-cellulose column. Based on the results of methylation analysis, partial acid hydrolysis and Smith degradation, the acidic xylan was concluded to be composed of a linear backbone of β-(1→4)-linked D-xylopyranosyl residues with approximately every ninth residue carrying a terminal 4-O-methyl-α-D-glucopyranosyluronic acid residue linked as a single side chain by (1→2) linkage.