Isolation of a Few Metabolites from p-Menthane and Conditions for cis-p-Menthan-1-ol Productrion by Pseudomonas Mendocina-SF

Abstract

The p-menthane-utilizing microorganism, strain-SF, was identified to be a strain belonging to Pseudomonas mendocina by the physiological and morphological properties. The productivity of cis-p-menthan-1-ol by this strain was examined under various conditions, and as a result it was found that this strain required both magnesium and ferric ions for the hydroxylation of p-menthane, and that the productivity increased up 20% to 35 or 40% by adding 0.1% (w/v) of agar or a proper emulsifier (PEG nonylphenylether p:10) into the medium. On the other hand, some new metabolites which were assumed to be the intermediates were identified to be 1-hydroxymethyl-4-isopropyl cyclohexanol, 1-carboxy-4-isopropyl cyclohexanol, and 3-isopropyl heptanedioic acid by IR, PMR, MS and elementary analyse. From these results, the methabolic pathway of p-menthane by strain-SF was proposed.