抄録
Lipolytic activities of intracellular lipase obtained from Streptococcus lactis 527 cells grown at 30°C were determined using bacterial neutral lipids extracted from cells grown at 10 and 30°C. The amounts of free fatty acids liberated from lipids by lipase were in the order: 30°C neutral lipid>10°C neutral lipid>triolein>intracellular membrane fraction. Glycerides hydrolyzed partially by lipase were detected on thin-layer plates and were composed of 1, 3-and 1, 2-diglycerides, fatty acids and unhydrolyzed triglycerides. Fatty acids liberated from neutral lipids by lipase were determined by gas chromatography. It was found that the major acid was cy-C19 and the minor Cl=18 among the acids liberated from 10°C neutral lipid, whereas the major acid was C1=18 and the minors C1=16 and cy-C19 from 30°C lipid.
