Abstract
Laminaran was isolated from Eisenia bicyclis. On the basis of the methylation, periodate oxidation, NMR spectroscopy by 1H and 13C nuclei, this glucan was shown to have a branched structure composed of (1→3)- and (1→6)-linked β-D-glucopyranose residues in the ratio 3:2. The glucan was especially characterized by comparison of its 13C and 1H NMR spectra with those of related compounds. The NMR investigation of the polyol polysaccharide, ob-tained by reduction after periodate oxidation of laminaran, was very useful for elucidation of the composition, structure and major sequence of the native glucan.
