Isolation and Identification of a Hog Pancreatic α-Amylase Inhibitor (Haim) Producing Streptomyces

Abstract

A screening test was carried out to obtain microbes which produce hog pancreatic α-amylase inhibitor and a new inhibitor was found in culture broth of an actinomycete, strain YM-25.This inhibitor was designated as Haim, an abbreviation for hog pancreatic α-amylase inhibitor from a microbe. The determined morphological and physiological properties of strain YM-25 led to the conclusion that the microorganism was Streptomyces griseosporeus.
When the microorganism was aerobically cultured at 30°C in a jar fermentor containing the most suitable medium for growth which consisted of 5% glycerol, 0.5% polypepton, 0.2% meat extract, 0.1% yeast extract, 0.4% Na₂HPO₄- 12H₂O, 0.1% KH₂PO₄, and 0.05% MgSO₄ - 7H₂O (pH 7.3), the highest activity of Haim was obtained on 23-26 hr cultivation.
Haim had specific inhibitory activities against animal α-amylases but not against microbial and plant α-amylases.