Thiobarbituric Acid Test for Detecting Lipid Hydroperoxides under Anaerobic Condition

Abstract

The thiobarbituric acid (TBA) test for detecting lipid hydroperoxides does not require for fomation of TBA-reacting compounds from hydroperoxides, but oxygen has an unfavorable effect, that is, it forms new hydroperoxides during the reaction when unoxidized lipids co-exist. Therefore, a method using a vacuum reaction tube was proposed.