Abstract
The effect of medium components and cultural conditions was studied on the production of polysaccharide (pendulan) and growth of mycelia in Porodisculus pendulus JTS-3009. Glucose, mannose, fructose, cellobiose, sucrose, mannitol and various starches were found to be suitable carbon sources. Pendulan was produced and mycelia grew well on ammonium-N, nitrate-N and organic nitrogen. The optimal concentration of nitrogen was 0.03 % for production of pendulan and 0.04 % for growth of mycelia. The addition of suitable amounts of yeast extract aided pendulan production and mycelium growth. The optimal temperature for pendulan production was about 29°C and for mycelium growth, 2530°C. The optimal pH for pendulan production was 68 and for mycelium growth, 56.5. The seed culture was stable after storage at 5°C or at -20°C with the addition of 2030 % (v/v) glycerin. The maximum yield of pendulan and mycelia was obtained at 90120 hr of fermentation.
