Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Factors Affecting the Surviving Fractions of Resting Escherichia coli B and K-12 Cells Exposed to Alternating Current
Keiko SHIMADAKenzo SHIMAHARA
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1981 年 45 巻 7 号 p. 1589-1595

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Resting Escherichia coli B and K-12 cells suspended in a phosphate buffer solution were exposed to alternating current (AC) of 50 Hz. The surviving fractions of cells exposed to AC were decreased with an increase in current density from 0 to 300mA/cm2 at 29±3°C for a definite exposure time. At a definite current density the fractions were decreased proportionally with exposure time. Even for conditions of definite exposure time and current density, the fractions varied depending on the concentration of cells initially suspended in buffer solution in AC-exposure chamber. E. coli cells were more resistant to AC-exposure under anaerobic conditions than aerobic conditions. Addition of catalase to the cell suspension during AC-exposure effectively protected both strains. Methionine and albumin had mild protective effects, but chemicals, such as cysteine, ehanol or a-tocopherol had little protective effect.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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