Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
L-Methionine Production by Ethionine-resistant Mutants of a Facultative Methylotroph, Pseudomonas FM 518
Yasushi morinagaYoshiki taniHideaki YAMADA
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1982 年 46 巻 2 号 p. 473-480

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Washed cells of facultative methylotrophs which have the serine pathway showed high activities for L-methionine formation from DL-homocysteine, in the presence of methanol as methyl donor. Strain FM 518, isolated from soil and identified as a bacterium belonging to the genus Pseudomonas, showed the highest activity for L-methionine formation and was used as the parental strain for breeding the L-methionine-producing mutants. An ethionine-resistant mutant, FE 244, derived from strain FM 518, accumulated 0.8 mg/ml L-methionine in a methanol-medium under optimum conditions.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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