DNA Breakage by Hydrolyzable Tannins in the Presence of Cupric Ion

Sanetaka SHIRAHATA; Hiroki MURAKAMI; Kazuo NISHIYAMA; Itsuro SUGATA; Kazuki SHINOHARA; Gen-ichiro NONAKA; Itsuo NISHIOKA; Hirohisa OMURA

doi:10.1271/bbb1961.49.1033

Sanetaka SHIRAHATA, Hiroki MURAKAMI, Kazuo NISHIYAMA, Itsuro SUGATA, Kazuki SHINOHARA, Gen-ichiro NONAKA, Itsuo NISHIOKA, Hirohisa OMURA

著者情報

ジャーナルフリー

1985 年 49 巻 4 号 p. 1033-1040

DOI https://doi.org/10.1271/bbb1961.49.1033

詳細

抄録

DNA breakage by hydrolyzable tannins of known structures was investigated by agarose or polyacrylamide gel electrophoretic analysis. Hydrolyzable tannins could cause both double-strand and single-strand breakages in λDNA in the presence of Cu²⁺. The breaking reaction was strongly suppressed at concentrations higher than 100μM of hydrolyzable tannins. DNA breakage by various tannins was dependent upon their sorts, numbers, and binding sites of the constituent acids and polyalcohols. Metallic ions other than Cu²⁺ had little effect on the breaking reaction.

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