抄録
Components of the Na+-motive NADH: quinone oxidoreductase segment in the respiratory chain of Vibrio alginolyticus were examined in membranes prepared from wild type, Na+-pump-defective mutants, and a spontaneous revertant. Ag+ distinguished two kinds of respiratory NADH dehydrogenases. The Na+-pump-defective mutants lacked Ag+-sensitive NADH dehydrogenase activity. Incubation of the Ag+-sensitive NADH dehydrogenase with solubilized membrane proteins of the mutant led to the reconstitution of Na+-motive NADH: quinone oxidoreductase activity. We think that Ag+-sensitive NADH dehydrogenase is an essential component of the respiratory Na+ pump of this organism.
