Abstract
Eight amylase inhibitors were isolated from a transglucosylation mixture of 1-deoxynojirimycin, soluble starch and bacterial saccharifying amylase. The inhibitors were found to be a series of compounds and were designated as (glucose)n • 1-deoxynojirimycin, from the results of constituent and reducing end analyses (n-1 to 8).
The linkage between glucoses was determined to be α-1, 4 by the permenthylation or β-amylase digestion method. The glucose-1-deoxynojirimycin linkage was determined to be α-1, 4 by 1H and 13C-NMR spectroscopy. The inhibitors inhibited various amylases depending on the number of glucose residues. The size of the active site (subsite) of an amylase might influence its susceptibility to the inhibitors.
