Effects of Nutritional and Hormonal Factors on the Formation of Anti-plant-viral Protein by Mirabilis jalapa L. Cells in Suspension Culture

抄録

To obtain basic information of the production of anti-plant-viral protein by Mirabilis jalapa cells in suspension culture, the cultural conditions, especially the nutritional ones, for MAP formation and cell growth were investigated. It was found MAP formation was greatly repressed by a high concentration of 2, 4-D and the addition of cytokinin. The concentration of nitrogen sources, phosphate, and CaCl₂ had marked effect. Revised medium was prepared due to the corresponding optimum concentration obtained from our investigations. MAP productivity in the revised medium became twice that in the basal medium. Changes in MAP content were also examined during successive subculturing. It was recognized that MAP content increased in the early generations, then decreased slowly during subculturings.