1987 年 51 巻 12 号 p. 3245-3249
The L-leucine productivity of an L-leucine producing strain, H-1204, of Corynebacterium glutamicum substantially decreased during a large-scale culture or repetitive subculturing. This instability was found to be due to the appearance of revertants with lower or no L-leucine productivity. Strains in the culture broth could be roughly classified into three types on the basis of their phcnotypes: L-type, original L-leucine producing strain, ValL Leu+ (valine leaky); M-type, Val+ Leu+ (prototroph); V-type, Val+ Leu- (leucine auxotroph). The appearance of these revertants was determined to be caused by the distribution imbalance of α-ketoisovaleric acid, the common precursor for L-leucine and L-valine biosynthesis.
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