Membrane-bound Cytochromes c of a KCN-resistant Mutant of an Obligate Methylotroph, Methylomonas sp.

抄録

The membrane-bound cytochromes c of an obligate methylotroph, Methylomonas sp. YK 1, and a KCN-resistant mutant, YK 56, were separated into two cytochrome c fractions, non-adsorbed and adsorbed fractions, on DEAE-cellulose column chromatography. The proportions of the non-adsorbed and adsorbed cytochromes c were 40 and 60%, respectively.
The membrane-bound cytochrome c of the mutant strain was purified by a procedure involving solubilization, and DEAE-cellulose, Cellulofme and Octyl-Sepharose column chromatographies. The Two purified membrane-bound cytochromes c had almost the same characteristics. Absorption peaks of the reduced forms of the two membrane-bound cytochromes c were seen at 551.5, 522.5, and 417.5nm at room temperature, and a shoulder on the β-band appeared at 530nm at low temperature. The molecular weight was calculated to be 10, 000 by high pressure liquid chromatography and the isoelectric point to be 4.1.