抄録
PNP-(GlcNAc)5, which is available as a substrate of a lysozyme assay, was efficiently synthesized by transfer of the tetra-N-acetyl-chitotetraosyl residue to the 4-position of PNP-GlcNAc using lysozyme in an aqueous solution containing DMSO in a high concentration. The use of an aqueous DMSO system in this reaction not only ensured a sufficient solubility of PNP-GlcNAc substrate, but also resulted in the high yield of PNP-(GlcNAc)5. PNP-(GlcNAc)5 insolubilized from the reaction mixture was directly obtained in a high state of purity by washing with aqueous methanol without using any column chromatographic separation.
