Induction and Purification of Aspergillus oryzae 1, 2-α-Mannosidase

Abstract

The culture conditions favoring the production and the subsequent purification of Aspergillus oryzae 1, 2-α-mannosidase were investigated. Baker's yeast mannan as the carbon source in liquid culture was found to induce 1, 2-α-mannosidase production but to depress protein and other glycosidase productions. Better enzyme production was achieved by developing a suitable cultivation medium containing baker's yeast mannan and a nitrogen source in proper concentrations. From the culture product thus obtained, 1, 2-α-mannosidase could be readily purified by the affinity chromatographic method previously developed [Tanimoto et al., J. Biochem., 99, 601 (1986)], without any other chromatographic step.