Cloning and Sequencing of Goat Growth Hormone Gene

Abstract

A clone that contains goat growth hormone (gGH) gene was isolated from a goat genomic library using bovine growth hormone cDNA as a probe. The gGH gene was located on a 5.8-kb EcoRI-HindIII fragment from the data of Southern hybridization analysis, and the gGH gene and its flanking region were completely sequenced. The nucleotide sequence of the region was highly homologous to those of other animals. The nucleotide sequence homologies of the promoter region of the gGH gene were 98% with the bovine, 90% with the porcine, 74% with the human, and 72% with the rat genes. The consensus sequence for the glucocorticoid receptor binding site was found in the first intron of the gGH gene. Comparison of the S'-flanking sequences of the gGH gene with those of other animals revealed that there were three homologous tracts among those sequences. We found conserved sequences in the 3'-flanking region.