Abstract
The substrate specificity of α-D-xylosidase from Bacillus sp. No. 693-1 was further investigated. The enzyme hydrolyzed α-1, 2-, α-1, 3-, and α-1, 4-xylobioses. It also acted on some heterooligosaccharides such as O-α-D-xylopyranosyl-(1→6)-D-glucopyranose, O-α-D-xylopyranosyl-(1→6)-O-β-D-glucopyranosyl-(1→4)-D-glucopyranose, O-α-D-xylopyranosyl-(1→6)-O-β-D-glucopyranosyl-(1→4)-O-[α-D-xylopyranosyl-(1→6)]-D-glucopyranose, and O-α-D-xylopyranosyl-(1→3)-L-arabinopyranose. The enzyme was unable to hydrolyze tamarinde polysaccharides although it could hydrolyze low molecular weight substrates with similar linkages.
