Purification of Endo-1, 4-β-glucanase Components from Fungal Strain Y-94

Abstract

Several endo-1, 4-β-glucanases [1, 4-β-D-glucan glucanohydrolase (EC 3.2.1.4)] from a fungus, Y-94, were purified to homogeneity by ammonium sulfate fractionation, column chromatography with two kinds of ion exchangers (Amberlite CG-50 and PBE 94), gel filtration with Toyopearl HM-55 and polyacrylamide gel electrophoresis, and their physicochemical and chemical properties were investigated. They differed in molecular weight (13, 000-174, 000), sugar content (around 16-20% and 0%), pI value (6.66-<3.8) and so on.