Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Cloning and Expression in Escherichia coli of Clostridium stercorarium Strain F-9 Genes Related to Xylan Hydrolysis
Kazuo SAKKAYuzo KOJIMAKazuhiro YOSHIKAWAKyo SHIMADA
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1990 年 54 巻 2 号 p. 337-342

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Nine distinct fragments of Clostridium stercorarium strain F-9 (which was isolated and identified in this laboratory) DNA have been cloned in Escherichia coli and shown to express enzymatic activities related to xylan hydrolysis. Two of the 9 E. coli clones showed xylanase activity, one showed β-xylosidase activity as well as xylanase activity, and the other 6 showed β-xylosidase activity. On comparing the restriction maps of the xylanase and xylosidase genes cloned in this study with those reported so far, it was concluded that all the 9 clones carried hitherto unidentified genes. All the enzymes expressed in the E. coli clones were thermophilic.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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