Abstract
A water-soluble derivative of rutin was found to be formed in a high yield from dextrin and rutin in 50% methanol by the successive actions of Bacillus stearothermophilus cyclomaltodextrin glucanotransferase and Rhizopus sp. glucoamylase. The derivative was isolated from the reaction mixture by paper chromatography, Toyopearl HW-40S column chromatography, lyophilization, and successive treatment with methanol and ethanol, and then obtained as yellowish needle crystals [232-235°C, decomp. 16D +65.3° (c = 3.77, H2O)]. The compound was identified as 4G-α-Dglucopyranosyl-rutin on the basis of the various experimental results, viz., UV, IR, 1H-NMR, and 13C-NMR spectra, products by hydrolysis with acid and with α- and β-glucosidases. The solubility of the compound in water was about 30×103 times higher than that of rutin.
