Analysis by Deletion and Site-directed Mutagenesis of Promoters of the Cell Wall Protein Gene Operon in Bacillus brevis 47

Abstract

The cell wall protein gene operon of Bacillus brevis 47 has multiple and tandem promoters. The precise locations of the two major promoters (P2 and P3) of the operon were determined by deletion analysis. This together with results of oligonucleotide-directed mutagenesis of the promoter regions confirmed that the -35 and -10 sequences of the two promoters proposed previously are essential for their promoter activity. A G + C-rich sequence upstream of the - 35 region of P2 and an A-rich sequence upstream of the - 35 region of P3 facilitate the transcription from P2 and P3, respectively.