1991 Volume 55 Issue 11 Pages 2745-2750
Two albumin fractions were extracted from defatted flours of seeds of Amaranthm hypochondriacus and A. cmentus. One fraction (Alb-1) was extracted with 0.5M NaCl or water when used as the first solvent. Another fraction (Alb-2) was extracted with water after removing Alb-1 and globulin. Apparent pIs of Alb-1 and Alb-2 were 4.5-7.5 and 5-6, respectively. Alb-2 was precipitated by various metal ions (1mM), while Alb-1 was not. Alb-2 contained four major components (Mr=56, 36, 26, 22 kD), although Alb-1 had no particular major proteins. Pronase treatment of amaranth flour before protein extraction decomposed Alb-1, but not Alb-2, as seen by SDS-PAGE analysis. The findings that Alb-2 was protected from the Pronase treatment and it could not be extracted until Alb-1 and globulin were removed suggests that Alb-2 might be associated with protein bodies [Irving et al., J. Food Sci., 46, 1170 (1981)].
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