Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Preparation of Triacylglycerol Molecular Species by Interesterification Using Endocellular Lipase in n-Hexane
Masami KIMURAKiyozo HASEGAWAHitoshi TAKAMURATeruyoshi MATOBA
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1991 Volume 55 Issue 12 Pages 3039-3043

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Abstract

The Interesterification of triacylglycerol with fatty acid was done to prepare triacylglycerol molecular species. Optimum operating conditions for the interesterification using a 1, 3-positional specific endocellular lipase from Rhizopus japonicus NR400 in a batch system were investigated. The reaction was done at 40°C for 5 hr in the following system: Trioleoylglycerol-palmitic acid=1:3.5 (mol/mol), 10ml n-hexane/g trioleoylglycerol, and 2500 units of enzyme/g trioleoylglycerol. Under these conditions, the content of palmitoyl groups in 1, 3-positions of triacylglycerol was about 60 mol%. Additional interesterification (2-cycle reaction) using palmitic acid and the novel triacylglycerol prepared by one-step interesterification (1-cycle reaction) resulted in a preparation of highly pure 1, 3-dipalmitoyl-2-oleoylglycerol.

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