Correlation between Retention and Hydrocarbonaceous Area of Non-protein Alicyclic α-Amino Acids in Reversed-phase HPLC

Abstract

The retention by reversed-phase HPLC of a homologous series of aliphatic amino acids increased as the size of the apolar moiety increased. By use of the hydrocarbonaceous surface area as the structural descriptor, we found that cyclic amino acids had higher retention indices than the corresponding linear homologues. The retention difference between ring and chain compounds probably arises from the entropy effects.