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Masanobu KOUTAKE, Ichiro MATSUNO, Hiroshi NABETANI, Mitsutoshi NAKAJIM ...
1992 Volume 56 Issue 5 Pages
697-700
Published: May 23, 1992
Released on J-STAGE: February 08, 2008
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To identify the cause of fouling of the membrane surface and pores during the ultrafiltration of whey or skim milk, these liquids were filtered through a membrane, allowed to adsorb to it, or both. Measurements of flux were made before and after gentle hand cleaning with a soft sponge, and resistance to permeation because of fouling was calculated and classified into four kinds : by adsorption to the surface, by adsorption in side the pores, because of filtration on the surface, and because of filtration in the pores. In the ultrafiltration of whey, resistance caused by fouling on the surface by filtration was great, and that caused by fouling in the pores by filtration was slight. In the ultrafiltration of skim milk, resistance caused by fouling by adsorption was great, and that caused by fouling on the surface by filtration was slight, probably because the casein micells in skim milk scrape fouling materials off the surface.
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Tamotsu EGUCHI, Yukihiro KUGE, Kunimi INOUE, Naohiro YOSHIKAWA, Kenich ...
1992 Volume 56 Issue 5 Pages
701-703
Published: May 23, 1992
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A new process for (6S)-tetrahydrofolate production from dihydrofolate was designed that used dihydrofolate reductase and an NADPH regeneration system. Glucose dehydrogenase from Gluconobacter scleroides KY3613 was used for recycling of the cofactor. The reaction mixture contained 200mM dihydrofolate, 220mM glucose, 2mM NADP, 14.4U/ml dihydrofolate reductase, and 14.4U/ml Glucose dehydrogenase, and the reaction was complete after incubation at pH 8.0, and 40°C for 2.5hr. With (6S)-tetrahydrofolate as the starting material, l-leucovorin was synthesized via a methenyl derivative. The purity of the l-leucovorin was 100%, and its diastereomeric purity was > 99.5% d.e. as the (6S)-form.
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Tsong-Rong YAN, Shih-Ching Ho, Chia-Lung Hou
1992 Volume 56 Issue 5 Pages
704-707
Published: May 23, 1992
Released on J-STAGE: February 08, 2008
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An X-prolyl dipeptidyl aminopeptidase (X-PDAP ; EC 3.4.14.5) was identified to be loosely bound on the inner cell membrane fraction of Lactococcus lactis subsp. cremoris nTR. The biosynthesis of X-PDAP was continuously increased before the late-log growth phase of the bacteria. Both Gly-Pro-pNA and Ala-Ala-pNA were hydrolyzed by X-PDAP ; the k
cat/K
m value of the former was about 10-fold that of the latter. The K
i of X-Pro and Pro-X were more specific to X-PDAP than those of X-Ala. The enzyme splitting a dipeptide sequentially from β-casomorphin as a model catalytic pattern was identified and some properties of the enzyme were further characterized.
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Toshio KIMURA, Isao SUGAHARA, Katsuyuki HANAI, Yuuko TONOMURA
1992 Volume 56 Issue 5 Pages
708-711
Published: May 23, 1992
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γ-Glutamylmethylamide synthetase [L-glutamate : methylamine ligase (ADP-forming), EC 6.3.4.12] was purified about 70-fold from a cell-free extract of Methylophaga sp. AA-30 by ammonium sulfate fractionation, Octyl-Sepharose column chromatography, and Sephacryl S-300 gel filtration. Only a single protein band was detected after SDS-polyacrylamide gel electrophoresis of the purified preparation ; the band was at a position corresponding to a molecular weight of 56, 000. The molecular weight of the enzyme was calculated to be 440, 000 by Superose 6HR gel filtration, so we suggest that the enzyme is an octomer of identical subunits. The enzyme had maximum activity at pH 7.5 and 40°C. It could use ethylamine and propylamine instead of methylamine as the substrate, but it could not use D-glutamate or L-glutamine instead of L-glutamate.
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Haruyuki KURODA, Shonosuke SAGISAKA
1992 Volume 56 Issue 5 Pages
712-715
Published: May 23, 1992
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When frozen flower buds of apple trees started to thaw, an abrupt decrease of levels of reduced glutathione and glucose-6-phosphate occurred, even without freezing injuries. With freezing injuries, a steep decrease in the levels of the above substrates was observed, suggesting that the level of generating peroxide depends on freezing temperatures. Enzyme systems involved in the regeneration of reduced glutathione also do not seem to function properly in poplar twigs in a frozen milieu. At about-10°C, a decrease in levels of reduced glutathione was detected and this decrease was followed by a decrease in levels of glucose 6-phosphate. When twigs that contained depressed level of reduced glutathione and glucose-6-phosphate were transferred to 4°C, overcompensation for the decrease in reduced glutathione and glucose-6-phosphate occurred and the resultant surplus appeared to reflect the metabolic dysfunction in the supply and demand for reduced glutathione and glucose-6-phosphate that occurs under frozen conditions. These results suggest that the sequence of reactions which starts from the reaction catalyzed by hexokinase, through reactions catalyzed by glucose-6-phosphate dehydrogenase and glutathione reductase, does not function properly under frozen conditions in perennial plants.
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Wataru KUGIMIYA, Yasuo OTANI, Mitsutaka KOHNO, Yukio HASHIMOTO
1992 Volume 56 Issue 5 Pages
716-719
Published: May 23, 1992
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Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.
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Il-Hwan LHO, Kohzo MORITA, Hiromichi MIYAKE, Kohji SAKAMOTO, Kohzo KAN ...
1992 Volume 56 Issue 5 Pages
720-724
Published: May 23, 1992
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The effect of iron (II)-ascorbate complex on various phages was investigated. At 10
-6 M, the complex inactivated all nine phages examined. The mechanism of the inactivation was studied with phage J1, the most sensitive to the complex. The addition of H
2O
2 or Cu
2+ to the reaction mixture increased the inactivation. Bubbling of nitrogen through the reaction mixture and the addition of Fe
3+, a reducing agent, a chelating agent, or a radical scavenger prevented inactivation. These findings suggest the involvement of oxygen radicals in the inactivation. The complex had no effects on the SDS-PAGE pattern or amino acid composition of bovine serum albumin, or the structural protein of phage J1. The complex nicked the supercoiled form of pUC18 DNA, giving first single-stranded breaks (the open circular form) and then double-stranded breaks (the linear form). Strands of M13mp8 DNA, λDNA, and J1 DNA were also broken. The breaks could account for the inactivation.
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Makoto YOSHIDA, Kaoru KOHYAMA, Katsuyoshi NISHINARI
1992 Volume 56 Issue 5 Pages
725-728
Published: May 23, 1992
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A new method to evaluate soybeans for making tofu (soybean curd) is proposed. Dynamic viscoelasticity measurements were carried out to examine the gelation process of soymilk, storage and loss moduli being observed as a function of time after adding glucono-δ-lactone. The gelation curves fitted well with first-order reaction kinetics. The saturated value of the storage modulus correlated well with gel hardness by a curdmeter. The saturated value of the storage modulus was mainly dependent on the concentration of 11S globulin, while the gelation rate was an increasing function of the concentration of glucono-δ-lactone at a fixed 11S globulin concentration.
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Shinzo KAGABU, Shigeo KAWAMURA, Tatsuya ASANO, Mari KANOH
1992 Volume 56 Issue 5 Pages
729-731
Published: May 23, 1992
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The retention by reversed-phase HPLC of a homologous series of aliphatic amino acids increased as the size of the apolar moiety increased. By use of the hydrocarbonaceous surface area as the structural descriptor, we found that cyclic amino acids had higher retention indices than the corresponding linear homologues. The retention difference between ring and chain compounds probably arises from the entropy effects.
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Takashi IDE, Masakazu MURATA, Hiroshi MORIUCHI
1992 Volume 56 Issue 5 Pages
732-735
Published: May 23, 1992
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Diets containing 10% soybean oil, soybean phospholipid and egg yolk phospholipid decreased rat liver microsomal diacylglycerol acyltransferase activity when measured with an endogenous diacylglycerol substrate relative to that in rats fed with a low-fat diet (0.5% soybean oil). Although no significant difference was detected in the enzyme activity among the rats fed with the diets containing 10% lipids, the extent of the decrease was more prominent with two kinds of phospholipids compared to soybean oil. When the enzyme activity was measured with an exogenous dioleoylglycerol substrate dispersed in ethanol, it was also significantly depressed by the diets containing 10% phospholipids and soybean oil, but to a much lesser extent. Dietary phospholipids reduced the microsomal concentration of diacylglycerol to a value less than one-third that in the animals fed with a low-fat diet. Soybean oil also decreased this parameter, but to a lesser extent. In addition, soybean and egg yolk phospholipids compared to soybean oil were more effective in reducing the triacylglycerol concentration in the liver. It seemed that dietary phospholipid compared to soybean oil exerted a more potent triacylglycerol lowering effect by modifying the concentration of microsomal diacylglycerol available for triacylglycerol synthesis in the liver.
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Toshiaki NAKAJIMA, Hiroo UCHIYAMA, Osami YAGI, Tadaatu NAKAHARA
1992 Volume 56 Issue 5 Pages
736-740
Published: May 23, 1992
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A soluble methane monooxygenase (sMMO : EC 1.14.13.25) was purified from a type II obligate methanotroph, Methylocystis sp. M. Ion exchange chromatography elution separated the sMMO into three components, I, II, and III. Components II and III were purified to homogeneity and were essential for the sMMO activity. Components II and III had molecular masses of approximately 233, 000 and 39, 000, respectively. Component II consisted of three subunits with molecular masses of 55, 000, 44, 000, and 21, 000, which appeared to be present in stoichiometric amounts, suggesting a (αβγ)
2 configuration in the native protein. Component II contained l-4 mol of iron and was considered to be a hydroxylase. Component III was a flavoprotein, which contained 1 mol of FAD as well as 1-2 mol of iron. It catalyzed the reduction of K
3Fe(CN)
6 and 2, 6-dichloroindophenol by NADH. Component I, which was partially purified and not essential for sMMO activity, stimulated the activity by about 11-fold. Its stimulation could be replaced by addition of Fe
2+. The molecular mass of the partially purified component I was estimated to be from 35, 000 to 40, 000 based on gel filtration, which suggested the presence of a new type of regulatory protein of sMMO.
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Alka B. TRIVEDI, Etsushiro DOI, Naofumi KITABATAKE
1992 Volume 56 Issue 5 Pages
741-745
Published: May 23, 1992
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Ochratoxin A was heated under three different moisture conditions, and under acidic and alkaline conditions. Heating to 175°C under the dry conditions produced little change in the molecule and cytotoxicity, detected by TLC and in the effects on the proliferation of HeLa cells. When heated under the moist and watery conditions, a small change in molecule was found by TLC, but cytotoxicity was not reduced. Under the acidic conditions (0.1 N HCl) the decomposition of ochratoxin A was detected by TLC, however the change in cytotoxicity was not observed in this assay system. On the other hand, heating with NaOH (0.1 N) resulted in the decomposition and detoxification of ochratoxin A. The HPTLC analysis showed the formation of some decomposed compounds including L-phenylalanine. This indicates the hydrolysis of ochratoxin A is one of the decomposition reactions induced by alkali at high temperatures.
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Kinji MIYASHITA, Mikiko KUSUMI, Ryutaro UTSUMI, Tohru KOMANO, Nobukats ...
1992 Volume 56 Issue 5 Pages
746-750
Published: May 23, 1992
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We have cloned various lengths of coxsackievirus B3 cDNA encompassing the region encoding the 3C proteinase, which is essential to the viral replication cycle. Such viral cDNAs were fused in frame to the 5'terminal portion of the lacZ' gene carried on the vector pUCll8 to express mature 3C proteinase in Escherichia coli. In the E. coli cells containing pCXB108 or pCXB117, constructed for this study, a large amount of 23-kDa protein was synthesized in the presence of IPTG. This protein was purified and was shown to be intact 3C proteinase. These data suggest that 3C proteinase, expressed as a part of a fusion protein, was active in E. coli and released itself from the precursor fusion protein by autocatalytic cleavage.
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Yoshiharu MIURA, Chikashi SAITOH, Shinjirou MATSUOKA, Kazuhisa MIYAMOT ...
1992 Volume 56 Issue 5 Pages
751-754
Published: May 23, 1992
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Stably sustained continuous production of hydrogen with high molar yield was achieved through a combination of dark fermentative hydrogen evolution by Chlamydomonas sp. strain MGA161 and hydrogen photoevolution by a marine photosynthetic bacterium W-1S in an alternating light-dark cycle as a model of the day-night cycle. The newly isolated stain W-1S could use acetic acid and ethanol excreted by strain MGA161 as electron donors for hydrogen photoevolution. The fermentation broth of stain MGA161 stimulated the hydrogen photoproduction of strain W-1S. This alga-bacterial combination had a high conversion yield of 8 mol H
2/mol of glucose of starch, with the possibility of improvement up to 10.5.
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Keiichi Aso, Hiroaki KODAKA
1992 Volume 56 Issue 5 Pages
755-758
Published: May 23, 1992
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The oligomerization of L-lysine esters with a free α-amino group was done using trypsin as a catalyst in aqueous media, and some reaction parameters were examined. The pH-dependence of the reaction suggested that aminolysis by the substrate species having non-protonated α- and ε-amino groups was a dominant factor governing the reaction progress. At the beginning of the reaction lysine oligomers containing up to 8 residues were observed, but much of the dimer was accumulated during prolonged incubation due to secondary hydrolysis of highly polymerized products. The reaction yield depended on the ratio of enzyme and substrate ; an overall reaction yield higher than 70% was attained after 2hr of reaction when 1, 000 mM of L-lysine ethyl ester was treated with 200 μM of trypsin at pH 10.0. The oligomerization was apparently enhanced by an addition of NaCl and by using the longer alkyl esters.
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Nobuji NAKATANI, Mayumi NAGASHIMA
1992 Volume 56 Issue 5 Pages
759-762
Published: May 23, 1992
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A main pungent amide, spilanthol (1), and three alkamides, (2E)-N-(2-methylbutyl)-2-undecene-8, 10-diynamide (2), (2E, 7Z)-N-isobutyl-2, 7-tridecadiene-10, 12-diynamide (3), and (7Z)-N-isobutyl-7-tridecene-10, 12-diynamide (4) were isolated from the flower heads of Spilanthes acmella L. var. oleracea Clarke. Their structures were established by spectroscopic methods. Compounds 2 and 4 were new and 3 was found for the first time in Spilanthes species. Chemotaxonomic aspects are discussed.
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Kazuhiro TOMITA, Toshihide NAKANISHI, Yoshiyuki KURATSU
1992 Volume 56 Issue 5 Pages
763-765
Published: May 23, 1992
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The effect of osmotic strength on 5'-inosinic add (IMP) fermentation was examined with a mutant, Corynebacterium ammoniagenes KY10895. The growth of KY10895 was inhibited by increased osmotic strength in the medium, but the specific IMP production (IMP produced/cell density) was promoted. The concentration of L-proline increased greatly under osmotic stress. Mutants resistant to growth inhibition by osmotic strength were derived from KY10895. Strain H-4415 had the highest IMP productivity. The intracellular concentration of L-proline in strain H-4415 was higher than in the parental strain. The addition of an osmoprotectant, betaine, in the production medium did not increase IMP production. These results suggested that the derivation of an osmotolerant stain is an effective method to obtain a hyperproducer of IMP, and that an increase in intracellular L-proline is related to increased IMP production.
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Shoichiro SAWAMURA, Yasuhiro TONOSAKI, Shigeyuki HAMADA
1992 Volume 56 Issue 5 Pages
766-768
Published: May 23, 1992
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Ellagic acid and its derivatives were found to abrogate the enzymatic activity of the glucosyltransferases (GTases) of mutans streptococci. These compounds preferentially inhibited the water-soluble glucan synthesis by GTase of Streptococcus mutans MT8148 and inhibited the water-insoluble glucan synthesis by GTase of Streptococcus sobrinus 6715. This inhibition resulted in a reduction of cellular adherence of mutans streptococci to a glass surface.
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Akira MURAKAMI, Shizuko TANAKA, Hajime OHIGASHI, Mitsuru HIROTA, Ryozo ...
1992 Volume 56 Issue 5 Pages
769-772
Published: May 23, 1992
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Two chalcone tetramers were isolated as inhibitors of Epstein-Barr virus (EBV)-activation induced by a tumor promoter, teleocidin B-4, from a medicinal plant in tropical west Africa, Lophira alata (Ochnaceae). One of them was identified as lophirachalcone. The other, named alatachalcone, was new, and the structure was determined by spectral properties. Both compounds also showed potent inhibitory activities against teleocidin B-4-induced inflammation on mouse ear. In an initiation-promotion experiment on mouse skin, alatachalcone (16nmol) significantly inhibited tumor promotion caused by 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6nmol).
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Takamitsu YORIFUJI, Eiichi SHIMIZU, Hiroshi HIRATA, Kan IMADA, Toshiak ...
1992 Volume 56 Issue 5 Pages
773-777
Published: May 23, 1992
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Guanidinobutyrase (guanidinobutyrate amidinohydrolase, EC. 3.5.3.7) catalyzing the third step of the arginine oxygenase pathway in Brevibacterium helvolum IFO 12073 (ATCC 11822) was purified to homogeneity and characterized. The enzyme had a molecular weight of 190, 000 and was composed of four apparently identical subunits with a molecular weight of 45, 000. The E(1%) value at 280nm of the enzyme protein was 2.4. The enzyme contained 0.5 mol of firmly bound Zn
2+ per mol of subunit. The enzyme was highly specific for 4-guanidinobutyrate, but had a weak activity toward L-and D-arginine. The Michaelis constant (K
m) for 4-guanidinobutyrate was 2.9 mM. The optimum pH was 9.0. Strong mixed type inhibition was observed with thioglycolate and several other thiol compounds. These properties were compared with those of the enzyme of fluorescent Pseudomonas and discussed.
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Motonori TSUJI, Eiichi KUWANO, Tetsuya SAITO, Morifusa ETO
1992 Volume 56 Issue 5 Pages
778-782
Published: May 23, 1992
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A number of N-acyl-L-proline derivatives were synthesized and their biological activities were investigated by using lettuce (Lactuca sativa L. cv. Sacramento) seedling test. A wide variety of these compounds promoted root growth at 25°C both under light and in darkness. Of the compounds tested, N-(2-fluorobenzoyl)-L-proline methyl ester (4) showed the highest activity and caused a 270% increase in the root elongation compared to the control. N-(2-Naphthoyl)-L-proline methyl ester (14) promoted the root growth, while N-(1-naphthoyl)-L-proline methyl ester inhibited it. L-Proline, benzoic acid, and 2-naphthoic acid had no significant effect on lettuce seedlings. Compounds 4 and 14, and N-(2-chlorobenzoyl)-L-proline methyl ester (7) reduced the inhibitory effect of 1 ppm ABA on the root growth, while the D-isomer of 4 was less activite than compound 4. Compounds 4, 7, and 14 did not show any rescue-activity for the complete inhibition of germination that was caused by treating 10 ppm of ABA.
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Katsumi SHIBATA, Miyuki SHIOTANI, Michiko ONODERA, Takeshi SUZUKI
1992 Volume 56 Issue 5 Pages
783-787
Published: May 23, 1992
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Effects of a threonine-, tryptophan-, aspartic acid-, lysine-, leucine-, or methionine-free diet fed to rats on the metabolism of nicotinamide were investigated. The body weights of rats and food intakes were greatly decreased by feeding of the diet excluding any of the above essential amino acids compared to the control group, however, not by feeding of an aspartic acid-free diet. The sum of the urinary excretion of nicotinamide, N
1-methylnicotinamide (MNA), N
1-methyl-2-pyridone-5-carboxamide (2-Py), and N
1-methyl-4-pyridone-3-carboxamide (4-Py) changed roughly in proportion to food intake. In the groups fed with the threonine-and lysine-free diets, the urinary excretion of MNA greatly increased compared with the control group during the whole experimental period and in the groups fed with the leucine-and methionine-free diets, increased excretion of MNA was observed on day 0-day 1. Whenever the increase in MNA excretion was observed, a decrease in 4-Py excretion was observed. This was attributed to the activity of 4-Py-forming MNA oxidase decreasing when rats were fed with the diet excluding one of the essential amino acid except for tryptophan. Therefore, the (2-Py+4-Py)/MNA excretion was greatly decreased by feeding of the diet excluding one of the essential amino acids except for the tryptophan-free diet. These results strengthened our hypothesis that the (2-Py+4-Py)/MNA excretion reflects the adequacy of amine acid nutrition.
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Yutaka OGURA, Miho TAKEMURA, Kenji ODA, Katsuyuki YAMATO, Eiji OHTA, H ...
1992 Volume 56 Issue 5 Pages
788-793
Published: May 23, 1992
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A gene, frxC, which is unique to the chloroplast genome of the liverwort Marchantia polymorpha, has sequence similarity to nifH, the product of which is an iron protein of a nitrogenase. Although frxC is expressed to produce a protein in liverwort chloroplasts, its function is not known. Using a probe of liverwort chloroplast DNA, a 10.1-kb region containing a gene cluster consisting of open reading frames (ORF278-frxC-ORF469-ORF248) was isolated from the cyanobacterium Synechocystis PCC6803. In this region, frxC and ORF469 showed sequence similarities to liverwort chloroplast frxC (83%) and immediately downstream ORF465 (74%), respectively. Synechocystis frxC showed 31% amino acid sequence identity with nifH1 from Clostridium pasteurianum. Additionally, Synechocystis ORF469 showed a sequence similarity (19% identity) to C. pasteurianum nifK product, which is the β subunit of a molybdenum-iron protein of a nitrogenase complex. Conservation of the gene arrangement between liverwort and Synechocystis suggests that the liverwort chloroplast frxC-ORF465 cluster may have evolved from an ancestor common to Synechocystis, and that these two genes may have been transferred to the nuclear genome in tobacco and rice during evolution.
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Akira TACHIKI, Hideya FUKUZAWA, Shigetoh MIYACHI
1992 Volume 56 Issue 5 Pages
794-798
Published: May 23, 1992
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From high-CO
2 (5% CO
2) grown unicellular green alga, Chlamydomonas reinhardtii, carbonic anhydrase (CA) was isolated by affinity chromatography and characterized. Isolated CA was identified as an isozyme (CA2) which is the product from the second gene CAH2 by peptide sequencing. The CA2 was inactivated by dithiothreitol. This treatment caused dissociation of CA2 into the large (38 kDa) and small subunits (4243 Da). The molecular mass of the CA2 holoenzyme measured by low-angle laser light-scattering photometry and precision differential refractometry combined with gel-filtration HPLC was 87.9 kDa. These results and gene structure indicate that CA2 is a heterotetramer consisting of two large and two small subunits linked by disulfide bonds like CA1, which is the CAH1 gene product. The specific activity of CA2 purified by anion-exchange HPLC was 3300 units per mg protein, which was approximately 1.6 times higher than that of CA1. Therefore, it was concluded that two structurally related isozymes, CA1 and CA2, are present in the wild type cells of C. reinhardtii and differentially regulated by the atmospheric CO
2 concentration.
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Yi-Chang KER, Rong-Huei CHEN, Ching-Shyong WU
1992 Volume 56 Issue 5 Pages
799-800
Published: May 23, 1992
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Kyu Beom KIM, Shiro KISHIHARA, Satoshi FUJII
1992 Volume 56 Issue 5 Pages
801-802
Published: May 23, 1992
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Renni R. SAMSOEDIN, Manabu ODASHIMA, Kazuhisa SUDO, Tsunehiro YAGOSHI, ...
1992 Volume 56 Issue 5 Pages
803
Published: May 23, 1992
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Koji TADASA, Ichizo SHIMODA, Hiroshi KAYAHARA
1992 Volume 56 Issue 5 Pages
804-805
Published: May 23, 1992
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Masaki NINOMIYA, Toshiake MATSUZAKI, Hitoshi SHIGEMATSU
1992 Volume 56 Issue 5 Pages
806-807
Published: May 23, 1992
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Toshiya TAKANO, Akira MIYAUCHI, Hiroaki TAKAGI, Kiyoshi KADOWAKI, Kuni ...
1992 Volume 56 Issue 5 Pages
808-809
Published: May 23, 1992
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Tadahiro NAGATA, Yasuo ANDO, Akira HIROTA
1992 Volume 56 Issue 5 Pages
810-811
Published: May 23, 1992
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Shogo EBISU, Hiroaki TAKAGI, Kiyoshi KADOWAKI, Hideo YAMAGATA, Shigezo ...
1992 Volume 56 Issue 5 Pages
812-813
Published: May 23, 1992
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Kuniji TANAKA, Tatsuya AKAI
1992 Volume 56 Issue 5 Pages
814-815
Published: May 23, 1992
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Nobuhiro FUKUDA, Kumiko HIOKI, Tetsuhiro ETOH, Toshiro HIDAKA, Ikuo IK ...
1992 Volume 56 Issue 5 Pages
816-817
Published: May 23, 1992
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Takashi EBATA, Hiroshi KAWAKAMI, Koshi KOSEKI, Katsuya MATSUMOTO, Haji ...
1992 Volume 56 Issue 5 Pages
818-819
Published: May 23, 1992
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Michihiko KATAOKA, Yoshiko NOMURA, Sakayu SHIMIZU, Hideaki YAMADA
1992 Volume 56 Issue 5 Pages
820-821
Published: May 23, 1992
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Ava HOLAZO, Hirofumi SHINOYAMA, Yoshi KAMIYAMA, Tsuneo YASUI
1992 Volume 56 Issue 5 Pages
822-824
Published: May 23, 1992
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Katsumi SHIBATA, Junko SUMIZAKI, Michiko ONODERA
1992 Volume 56 Issue 5 Pages
825
Published: May 23, 1992
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Kazuo KANATANI, Takatsugu TAHARA, Kazushi YOSHIDA, Hirosumi MIURA, Mas ...
1992 Volume 56 Issue 5 Pages
826-827
Published: May 23, 1992
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Hiroshi KUMAGAI, Kumi NAKAMOTO, Masaki NAKAMURA, Seiyu HIROSE, Yoshiko ...
1992 Volume 56 Issue 5 Pages
828-830
Published: May 23, 1992
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Hiroaki SAITO, Miho UDAGAWA
1992 Volume 56 Issue 5 Pages
831-832
Published: May 23, 1992
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Tomoyuki OKAMOTO, Katsumi NAKAMURA
1992 Volume 56 Issue 5 Pages
833
Published: May 23, 1992
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Suguru TAKATSUTO, Hiroki ABE
1992 Volume 56 Issue 5 Pages
834-835
Published: May 23, 1992
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Yoshihiko OZAKI, Shigeru AYANO, Masaki MIYAKE, Hisao MAEDA, Yasushi IF ...
1992 Volume 56 Issue 5 Pages
836-837
Published: May 23, 1992
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Toru HASEGAWA, Atsushi KAMADA, Kenji MORI
1992 Volume 56 Issue 5 Pages
838-839
Published: May 23, 1992
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Naoyuki FUKUCHI, Kazuo FURIHATA, Seiji TAKAYAMA, Akira ISOGAI, Akinori ...
1992 Volume 56 Issue 5 Pages
840-841
Published: May 23, 1992
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Rikizo AONO, Michizane HASHIMOTO, Atsushi HAYAKAWA, Satoshi NAKAMURA, ...
1992 Volume 56 Issue 5 Pages
842-844
Published: May 23, 1992
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Mitsuo JISAKA, Masanori KAWANAKA, Hiromu SUGIYAMA, Kazunori TAKEGAWA, ...
1992 Volume 56 Issue 5 Pages
845-846
Published: May 23, 1992
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