Site-directed Mutagenesis of Chitinase from Alteromonas sp. Strain O-7

Hiroshi Tsujibo; Hideyuki Orikoshi; Chiaki Imada; Yoshiro Okami; Katsushiro Miyamoto; Yoshihiko Inamori

doi:10.1271/bbb.57.1396

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Site-directed Mutagenesis of Chitinase from Alteromonas sp. Strain O-7

Hiroshi Tsujibo, Hideyuki Orikoshi, Chiaki Imada, Yoshiro Okami, Katsushiro Miyamoto, Yoshihiko Inamori

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JOURNAL FREE ACCESS

1993 Volume 57 Issue 8 Pages 1396-1397

DOI https://doi.org/10.1271/bbb.57.1396

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Abstract

Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of β-glycosidic linkage of chitin.

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