Abstract
We developed a method for the fractionation and purification of antitumor polysaccharides, considered to be a type of immunopotentiator or BRM (biological response modifier), from the mycelium of liquid cultured Grifola frondosa. The active polysaccharide fractions that showed higher antitumor activity were considered to be heteroglycans or their protein complexes as follows, in water-soluble fractions : FI0-a-α : fucogalactomannan-protein complex ; FI0-a-β : mannogalactofucan ; FA-1 : galactoglucomannofucan-protein complex ; FA-2-b-α : glucogalactomannan-protein complex ; in water-insoluble fractions : FIII-1-a : mannofucoglucoxylan ; FIII-1-b : mannoglucofucoxylan-protein complex ; FIII-2-a : mannofucoglucoxylan-protein complex ; FIII-2-b : glucomannofucoxylan-protein complex.
