Author's Organization：Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
In tobacco cell culture (Nicotiana tabacum L. "Bright Yellow"T-13), phenylalanine ammonia-lyase (PAL) activity was induced in response to an exogenously added kinetin. RNA blot hybridization analysis showed that a single species of PAL transcript 2. 9-kb in size was detected using the PAL cDNA cloned from kinetin-treated cells. The cellular content of this transcript increased transiently (2. 5-fold) 4h after the addition of kinetin followed by an increase in PAL enzyme activity at 16h.
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