Identification of Trisaccharide Xy1α1→3Xy1α1→3Glc in Human Urine

Abstract

Oligosaccharides in human urine were converted to pyridylamino(PA)-derivatives. From the PA-oligosaccharides, a saccharide thatwas chromatographically identical with a synthetic standard, Xyl-α1→3Xylα1→3Glc-PA, was isolated by gel filtration and HPLC. Structure analysis showed that the saccharide was Xylα1→3Xyl-α1→3Glc-PA. It is likely that Xylα1→3Xylα1→3Glc originated from such glycoconjugates as blood coagulation factors VII and IX, and protein Z.