Abstract
To analyze the primary structure of Mortierella vinacea α-galactosidase, a cDNA library of M. vinacea mRNA in λgt10 was constructed. A clone, which has an insert size of about 1.4 kilobase pairs, was found to contain the coding region of the mature enzyme. The deduced amino acid sequence showed that the mature enzyme consisted of 397 amino acid residues with a molecular mass of 44, 350Da. The sequence identity of the mature enzyme with α-galactosidases from Saccharomyces carlsbergensis, Cyamopsis tetragonoloba (guar), and human were 47%, 43%, and 34%, respectively.
