Determination by ¹H-NMR of the Stereospecificity of NAD-dependent Plant L-Histidinol Dehydrogenase for Nicotinamide C-4 Hydrogen Transfer

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The hydrogen-transfer stereospecificity of cabbage histidinol dehydrogenase at the C-4 position of NAD⁺ was determined by means of ¹H-NMR. A dehydrogenase reaction with enzymatically prepared [4-²H]NAD⁺ was performed. The NMR spectrum of the reaction mixture showed a peak at about 2.8 ppm, indicating the production of [(4S)-²H]NADH, indicating that the stereospecificity of the enzyme was pro-R-specific.