Random Attack as the Hydrolytic Reaction Mode of Oligosaccharides by Rhizopus Glucoamylase
1995 年 59 巻 7 号 p. 1372-1373
詳細
1995 年 59 巻 7 号 p. 1372-1373
Hydrolytic reactions of maltooligosaccharides (degree of polymerization n=3-7) and isomaltooligosaccharides (n=3-7) by Rhizopus glucoamylase (at pH 4.5, 25°C) were observed by HPLC. It was confirmed that initial rates of the decrease of n-mer substrate and the increase of the products, glucose and (n-1)-mer oligosaccharide, are the same for all the substrates, indicating that the reaction mode of the enzyme is "random attack, " and no "multiple attack" or "single chain attack" is operating.
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