Specific Proline and Hydroxyproline Detection Method by Post-column Derivatization for High-performance Liquid Chromatography
1995 年 59 巻 9 号 p. 1764-1765
詳細
1995 年 59 巻 9 号 p. 1764-1765
A specific detection method for hydroxyproline by post-column derivatization with 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole was developed after chiral resolution by ligand-exchange chromatography. The addition of ethylenediaminetetraacetic acid to the reaction mixture was effective to achieve the post-column reaction. The calibration curve for trans-4-hydroxy-L-proline shows linearity from 7. 6pmol up to 7600pmol.
この記事は最新の被引用情報を取得できません。
