Stable Isotope Labeled Cytochrome c₃ from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

Abstract

The conditions of cultivation and the composition of medium for Desulfovibrio vulgaris Miyazaki F (DvMF) were examined to obtain cytochrome c₃ labeled with a stable isotope. The growth of DvMF was steady and reproducible under purging with N₂ and under pH control. DvMF was able to grow on a defined medium without natural products. The composition of medium containing a small amount of NH₄Cl as sole nitrogen source was established. Then, [U-¹⁵N]cytochrome c₃ was obtained during the culture of DvMF in a defined medium with ¹⁵NH₄Cl; it was confirmed by ¹H-¹⁵N HMQC. This is the first report of [U-¹⁵N]cytochrome c₃.