Abstract
Nigrosporin A and B, two new phytotoxic and antibacterial metabolites were isolated from a culture filtrate of Ngrospora oryzae. The active principles were absorbed on XAD-2 resin and purified by suceessive ODS-HPLC. The structures were identified by spectroscopic and derivatization analysis as naphthoquinone derivatives. The substances showed phytotoxic activities, such as root elongation inhibition, necrotic effects, oxygen evolution inhibition, starch synthesis inhibition, and CO2 fixation inhibition at concentrations of 10-100 ppm. They also showed growth inhibition activity against Bacillus subtilis in a disc diffusion assay as well as when compared with streptomycin.
