Amino Acid Sequence and Characterization of Aldo-keto Reductase from Bakers' Yeast

Abstract

One of the enzymes from bakers' yeast that catalyzes the reduction of α- and β-keto esters has been studied. The N-terminal amino acid sequence of the enzyme shows that the enzyme belongs to the aldo-keto reductase superfamily. The substrate specificity of the enzyme is broad and resembles those of other aldo-keto reductases. The enzyme catalyzes the reduction of keto esters, aldehydes, and aldohexoses.