Purification and Characterization of the NADP-Malic Enzyme from Bradyrhizobium japonicum A1017

Fan CHEN; Youichi OKABE; Kaoru OSANO; Shigeyuki TAJIMA

doi:10.1271/bbb.61.384

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Purification and Characterization of the NADP-Malic Enzyme from Bradyrhizobium japonicum A1017

Fan CHEN, Youichi OKABE, Kaoru OSANO, Shigeyuki TAJIMA

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Keywords: Bradyrhizobium japonicum, NAD, NADP, malic enzyme, symbiotic nitrogen fixation

JOURNAL FREE ACCESS

1997 Volume 61 Issue 2 Pages 384-386

DOI https://doi.org/10.1271/bbb.61.384

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Abstract

An NADP-malic enzyme [EC 1.1.1.40] was purified to homogeneity from Bradyrhizobium japonicum A1017, and the molecular and physiological characteristics were surveyed. The molecular mass of one subunit of the purified enzyme was evaluated to be 77, 600 Da by SDS-PAGE, and the native enzyme was a tetramer in pH 7.0 and dimer in pH 8.0 conditions, showing complex oligomeric characteristics corresponding to pH value.

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