Bifidobacteria and Microflora
Online ISSN : 1884-5126
Print ISSN : 0286-9306
ISSN-L : 0286-9306
An Automatic System for Enumeration of Colony-Forming Units by the Surface Plate Method
Yoshimi BENNOShigenari KUKISAKITomotari MITSUOK
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ジャーナル フリー

1989 年 8 巻 2 号 p. 87-94

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抄録
A device for mechanical dilution and application of specimens and counting microbial colonies was developed. The device consists of a transport apparatus which transports petri dishes from one side of the apparatus to the other in such a way that the plate and lid of petri dishes are entirely separated at a particular stage. A diluting and inoculating system performs 10-fold dilutions of specimens from 10-1 to 10-8 dilutions and inoculation of the diluents. The inoculum is applied to the agar surface of preselected petri plates by an application system. Both a diluting and inoculating system and an application system can be connected to the apparatus. In this way a maximum of 25 agar plates can be handled. This new automatic system for enumeration of colony-forming units by the surface plate method is more accurate and reliable than the customary conventional methods.
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