The Influence of Intestinal Bacteria on Wound-Healing in Mice

抄録

The role of some intestinal bacteria species on wound-healing in mice was studied by comparing male germ-free (GF) mice with their conventionalized (Cvz) and monoassociated counterparts harboring Lactobacillus reuteri (L), Bifidobacterium longum (BB), Escherichia coli (E), Bacteroides vulgatus (B) and Clostridium perfingens (C) species. An incision was made on the dorsal skin of each mouse. A tube containing a piece of sponge was then implanted subcutaneously to measure the hydroxyproline concentration in wound tissue fluid. Mice were killed on the 7th postoperative day. Mice in the Cvz, L and BB groups showed stronger break-strengths and higher concentrations of hydroxyproline than mice in the GF group. The lactic acid concentration in Cvz, L, and BB groups was much higher than that in GF, B and C groups. The amounts of SCFAs in the Cvz group was higher than those in the GF group. Concentrations of SCFAs in the L and BB groups were not significantly higher than the values in the GF group. These results showed that the wound-healing process could be enhanced by some intestinal bacteria, and that lactobacilli and bifidobacteria may play an important role in this enhancement. The data suggest that such enhanced wound-healing in mice carrying special types of bacteria was partly due to the improvement in the nutritional status of mice caused by extra lactic acid produced by bacteria.