Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
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Quantitative Comparison of the Autofluorescence of Bacteria and Polystyrene Microspheres under Violet Wavelength Excitation for Verification of Fluorescence-based Bioaerosol Detector Results
NORIO HASEGAWA
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2013 年 18 巻 4 号 p. 211-215

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The autofluorescence intensity of bacteria and fungal spores was quantified by fluorescence microscopy in order to obtain the information for evaluating fluorescence-based bioaerosol detectors and was comparable to that of some types of polystyrene microspheres (PSMs). Although the intensity for microbes was distributed across a wide range over an order of magnitude in gray scale, it was in the intensity range of certain PSMs. Furthermore, some of those bacteria and PSMs were aerosolized in a test chamber and the fluorescence intensity was measured with a bioaerosol detector. Although there was a slight difference in the order of intensity from the results obtained by fluorescence microscopy, the fluorescence-based bioaerosol detector showed the intensity was in a comparable range.

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© 2013 The Society for Antibacterial and Antifungal Agents, Japan
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