抄録
1. D-Glucosamine deaminase activity of Escherichia coli K12 is markedly stimulated when the cells are grown in the medium containing D-glucose. It is concluded that this stimulatory effect is not due to the acidity brought about by fermentation of glucose, but due to the an-aerobiosis owing to fermentation gas formed during growth.
2. The degrees of stimulation are observed to be almost invariable for any level of the concentration of glucose between 0.25 and 2.0 per cent. The stimulation of the enzyme activity appears to be a temporary change in the organism.
3. The organism grown in the presence of glucose shows the highest activity of glucosamine deaminase when harvested at the time of its maximal growth (around the 16th hour of cultivation).
4. The presence of D-glucosamine, hexoses other than glucose, and diasccharides in the culture medium also exhibits stimulatory effect on the D-glucosamine deaminase activity.
The authors wish to express their thanks to Professor Senji Utzino, M. D., Kyoto University, for his interest and help to the present study.
